Review





Similar Products

spleen dissociation kit  (Miltenyi Biotec)
96
Miltenyi Biotec spleen dissociation kit
Spleen Dissociation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spleen dissociation kit/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
spleen dissociation kit - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
spleen cd11c n418  (Miltenyi Biotec)
96
Miltenyi Biotec spleen cd11c n418
FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse <t>(CD11c</t> cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.
Spleen Cd11c N418, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spleen cd11c n418/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
spleen cd11c n418 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
gentlemacs skin dissociation kit miltenyi biotec  (Miltenyi Biotec)
96
Miltenyi Biotec gentlemacs skin dissociation kit miltenyi biotec
FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse <t>(CD11c</t> cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.
Gentlemacs Skin Dissociation Kit Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gentlemacs skin dissociation kit miltenyi biotec/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
gentlemacs skin dissociation kit miltenyi biotec - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
enzyme mix  (Miltenyi Biotec)
96
Miltenyi Biotec enzyme mix
FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse <t>(CD11c</t> cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.
Enzyme Mix, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzyme mix/product/Miltenyi Biotec
Average 96 stars, based on 1 article reviews
enzyme mix - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
mouse spleen lymphocyte isolation solution kit  (Beijing Solarbio Science)
96
Beijing Solarbio Science mouse spleen lymphocyte isolation solution kit
FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse <t>(CD11c</t> cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.
Mouse Spleen Lymphocyte Isolation Solution Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse spleen lymphocyte isolation solution kit/product/Beijing Solarbio Science
Average 96 stars, based on 1 article reviews
mouse spleen lymphocyte isolation solution kit - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
mouse tumor mononuclear cell isolation kit  (Beijing Solarbio Science)
94
Beijing Solarbio Science mouse tumor mononuclear cell isolation kit
FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse <t>(CD11c</t> cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.
Mouse Tumor Mononuclear Cell Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse tumor mononuclear cell isolation kit/product/Beijing Solarbio Science
Average 94 stars, based on 1 article reviews
mouse tumor mononuclear cell isolation kit - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
mouse spleen lymphocyte isolation kit  (Beijing Solarbio Science)
96
Beijing Solarbio Science mouse spleen lymphocyte isolation kit
FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse <t>(CD11c</t> cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.
Mouse Spleen Lymphocyte Isolation Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse spleen lymphocyte isolation kit/product/Beijing Solarbio Science
Average 96 stars, based on 1 article reviews
mouse spleen lymphocyte isolation kit - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier

Image Search Results


FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse (CD11c cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.

Journal: Journal of Advanced Research

Article Title: The immune regulation and signaling transduction of FAM134B-mediated endoplasmic reticulum-phagy in ferroptosis of dendritic cells after sepsis

doi: 10.1016/j.jare.2025.07.058

Figure Lengend Snippet: FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse (CD11c cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.

Article Snippet: Spleen CD11c + (N418) and CD4 + T cell (L3T4) MicroBeads have been obtained from Miltenyi Biological GmbH (Bergisch Gladbach, Germany).

Techniques: Control, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Knock-Out